Ag Tech

Scientists develop ‘electronic nose’ to sniff out meat freshness

Beef Central, 11/11/2020


A team of scientists led by Nanyang Technological University, Singapore (NTU Singapore) has invented an artificial olfactory system that mimics the mammalian nose to assess the freshness of meat accurately.

The ‘electronic nose’ (e-nose) comprises a ‘barcode’ that changes colour over time in reaction to the gases produced by meat as it decays, and a barcode ‘reader’ in the form of a smartphone app powered by artificial intelligence (AI). The e-nose has been trained to recognise and predict meat freshness from a large library of barcode colours (See image 1 below).
When tested on commercially packaged chicken, fish and beef meat samples that were left to age, the team found that their deep convolutional neural network AI algorithm that powers the e-nose predicted the freshness of the meats with a 98.5 per cent accuracy. As a comparison, the research team assessed the prediction accuracy of a commonly used algorithm to measure the response of sensors like the barcode used in this e-nose. This type of analysis showed an overall accuracy of 61.7 per cent.
The e-nose, described in a paper published in the scientific journal Advanced Materials in October, could help to reduce food wastage by confirming to consumers whether meat is fit for consumption, more accurately than a ‘Best Before’ label could, said the research team from NTU Singapore, who collaborated with scientists from Jiangnan University, China, and Monash University, Australia.
Co-lead author Professor Chen Xiaodong, the Director of Innovative Centre for Flexible Devices at NTU, said: “Our proof-of-concept artificial olfactory system, which we tested in real-life scenarios, can be easily integrated into packaging materials and yields results in a short time without the bulky wiring used for electrical signal collection in some e-noses that were developed recently.
“These barcodes help consumers to save money by ensuring that they do not discard products that are still fit for consumption, which also helps the environment. The biodegradable and non-toxic nature of the barcodes also means they could be safely applied in all parts of the food supply chain to ensure food freshness.”
A patent has been filed for this method of real-time monitoring of food freshness, and the team is now working with a Singapore agribusiness company to extend this concept to other types of perishables.
A nose for freshness
The e-nose developed by NTU scientists and their collaborators comprises two elements: a coloured ‘barcode’ that reacts with gases produced by decaying meat; and a barcode ‘reader’ that uses AI to interpret the combination of colours on the barcode. To make the e-nose portable, the scientists integrated it into a smartphone app that can yield results in 30 seconds.
The e-nose mimics how a mammalian nose works. When gases produced by decaying meat bind to receptors in the mammalian nose, signals are generated and transmitted to the brain. The brain then collects these responses and organises them into patterns, allowing the mammal to identify the odour present as meat ages and rots.
In the e-nose, the 20 bars in the barcode act as the receptors. Each bar is made of chitosan (a natural sugar) embedded on a cellulose derivative and loaded with a different type of dye. These dyes react with the gases emitted by decaying meat and change colour in response to the different types and concentrations of gases, resulting in a unique combination of colours that serves as a ‘scent fingerprint’ for the state of any meat.
For instance, the first bar in the barcode contains a yellow dye that is weakly acidic. When exposed to nitrogen-containing compounds produced by decaying meat (called bioamines), this yellow dye changes into blue as the dye reacts with these compounds. The colour intensity changes with an increasing concentration of bioamines as meat decays further.
For this study, the scientists first developed a classification system (fresh, less fresh, or spoiled) using an international standard that determines meat freshness. This is done by extracting and measuring the amount of ammonia and two other bioamines found in fish packages wrapped in widely-used transparent PVC (polyvinyl chloride) packaging film and stored at 4°C (39°Fahrenheit) over five days at different intervals.
They concurrently monitored the freshness of these fish packages with barcodes glued on the inner side of the PVC film without touching the fish (See image 2 below). Images of these barcodes were taken at different intervals over five days.
E-nose achieves 98.5 per cent overall accuracy
A type of AI algorithm known as deep convolutional neural networks was then trained with images of different barcodes to identify patterns in the scent fingerprint that correspond to each category of freshness.
To gauge the prediction accuracy of their e-nose, the NTU scientists then monitored the freshness of commercially packed chicken, fish, and beef with barcodes glued on the packaging film, and stored at 25°C (77°Fahrenheit). Over 4,000 images of the barcodes from six meat packages were taken at different time intervals over 48 hours without opening the different meat packages.
The research team first trained their system to pick out patterns among the scent fingerprints captured in 3,475 barcode images, before testing the system’s accuracy on the remaining images.
The results revealed an overall 98.5 per cent accuracy – 100 per cent accuracy in identifying spoiled meats, and a 96 to 99 per cent accuracy for fresh and less fresh meats.
As a comparison, the research team randomly selected 20 barcode images from each freshness category to assess the prediction accuracy of Euclidean distance analysis, a commonly used method to measure the response of sensors like the barcode used in this e-nose. This analysis showed an overall accuracy of 61.7 per cent.
Prof Chen, President’s Chair Professor in Materials Science and Engineering at NTU, said: “While e-noses have been extensively researched, there are still bottlenecks to their commercialisation due to current prototypes’ issues with accurately detecting and identifying the odour. We need a system that has both a robust sensor setup and a data analysis method that can accurately predict scent fingerprints, which is what our e-nose offers.
“Its non-destructive, automated and real-time monitoring capability could also be used to recognise the types of gases that other types of perishable food emit as they become less fresh, providing a broadly applicable new platform for food quality control, which is what we are working towards now.”
E-nose 3.JPG
The e-nose comprises a ‘barcode’ that changes colour due to reactions with gases emitted by the decaying meat, and a barcode ‘reader’ in the form of a smartphone app powered by AI, and has been trained to recognise and predict meat freshness from a large library of barcode colours.
 E-nose 4.JPG
The barcode is attached to the underside of the PVC film that the meat is packaged in.
About Nanyang Technological University, Singapore
A research-intensive public university, Nanyang Technological University, Singapore (NTU Singapore) has 33,000 undergraduate and postgraduate students in the Engineering, Business, Science, Humanities, Arts, & Social Sciences, and Graduate colleges. It also has a medical school, the Lee Kong Chian School of Medicine, set up jointly with Imperial College London.
NTU is also home to world-class autonomous institutes – the National Institute of Education, S Rajaratnam School of International Studies, Earth Observatory of Singapore, and Singapore Centre for Environmental Life Sciences Engineering – and various leading research centres such as the Nanyang Environment & Water Research Institute (NEWRI) and Energy Research Institute @ NTU (ERI@N).
Ranked amongst the world’s top universities by QS, NTU has also been named the world’s top young university for the past seven years. The University’s main campus is frequently listed among the Top 15 most beautiful university campuses in the world and it has 57 Green Mark-certified (equivalent to LEED-certified) building projects, of which 95% are certified Green Mark Platinum. Apart from its main campus, NTU also has a campus in Singapore’s healthcare district.
Under the NTU Smart Campus vision, the University harnesses the power of digital technology and tech-enabled solutions to support better learning and living experiences, the discovery of new knowledge, and the sustainability of resources.
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  1. Cristina Delerue-Matos, 08/02/2021

    Good afternoon,
    This news is very interesting. How can I get more information about the electronic nose.
    Kind regards,

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